The information of SMCC(N-Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

SMCC(N-Succinimidyl4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is a actual popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide acknowledging groups at adverse ends of a medium-length cyclohexane spacer arm (8.3 angstroms).SMCC(N-Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is a water-soluble, non-cleavable and film closed crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters acknowledge with primary amines at pH 7-9 to anatomy abiding amide bonds. Maleimides acknowledge with sulfhydryl groups at pH 6.5-7.5 to anatomy abiding thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are almighty abiding up to pH 7.5 because of the cyclohexane arch in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is acrid up to ~10 mM in baptize and abounding frequently acclimated buffers, appropriately alienated the use of amoebic solvents which may adjy protein structure.Sulfo-SMCC is generally acclimated for the alertness of antibody-enzyme and hapten-carrier conjugates. In this blazon of conjugation, the NHS ester is reacted aboriginal with the antibody, balance crosslinking reagent removed and again the sulfhydryl-containing agitator atom is added. This two-step acknowledgment arrangement after-effects in accumulation of specific antibody-enzyme conjugates.SMCC(N-Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is the reagent of best for creating abiding maleimide-activated carrier proteins that will spontaneously acknowledge with sulfhydryls. In this application, the NHS ester of Sulfo-SMCC is reacted with lysine residues on the carrier protein, converting them to acknowledging maleimides. These almost abiding maleimide-activated intermediates may be lyophilized and stored for after alliance to a hapten.Applications for Sulfo-SMCC Ideal crosslinker for agitator labeling of antibodies, both agitator action and antibiotic specificity can be preserved Create specific bioconjugates via one- or two-step crosslinking reactions .Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens.
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