the information of SMCC(N-Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Product name:SMCC(N-Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Thermo Scientific Pierce SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide acknowledging groups at adverse ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms).
Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a non-cleavable and film absorbent crosslinker. It contains an amine-reactive N -hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters acknowledge with primary amines at pH 7-9 to anatomy abiding amide bonds. Maleimides acknowledge with sulfhydryl groups at pH 6.5-7.5 to anatomy abiding thioether bonds. The maleimide groups of SMCC and Sulfo-SMCC and are almighty abiding up to pH 7.5 because of the cyclohexane arch in the spacer arm.
SMCC Highlights
Amine acknowledging NHS ester crosslinks rapidly with primary amine-containing molecule
Sulfhydryl-reactive maleimide reacts with cysteine residues to crop specific conjugates
High purity, apparent SMCC can be acclimated to actualize high-purity maleimide-activated derivatives
Cyclohexane arch confers added adherence to the maleimide accumulation authoritative SMCC an ideal crosslinking abettor for maleimide activation of proteins. Maleimide groups are abiding for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.
Applications for SMCC
Ideal reagent for agitator labeling of antibodies, both agitator action and antibiotic specificity can be preserved
Create specific bioconjugates via one- or two-step crosslinking reactions
Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

SMCC is generally acclimated for the alertness of antibody-enzyme and hapten-carrier conjugates. In this blazon of conjugation, the NHS ester is reacted aboriginal with the antibody, balance crosslinking reagent removed and again the sulfhydryl-containing agitator atom is added. This two-step acknowledgment arrangement after-effects in accumulation of specific antibody-enzyme conjugates.
SMCC is a reagent of best for creating abiding maleimide-activated carrier proteins that will spontaneously acknowledge with sulfhydryls. In this application, the NHS ester of SMCC is reacted with lysine residues on the carrier protein, converting them to acknowledging maleimides. These almost abiding maleimide-activated intermediates may be lyophilized and stored for after alliance to a hapten.

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