Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a water-soluble, non-cleavable and film closed crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters acknowledge with primary amines at pH 7-9 to anatomy abiding amide bonds. Maleimides acknowledge with sulfhydryl groups at pH 6.5-7.5 to anatomy abiding thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are almighty abiding up to pH 7.5 because of the cyclohexane arch in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is acrid up to ~10 mM in baptize and abounding frequently acclimated buffers, appropriately alienated the use of amoebic solvents which may adjy protein structure.
Sulfo-SMCC Highlights
Amine acknowledging Sulfo-NHS ester crosslinks rapidly with primary amine-containing atom
Sulfhydryl-reactive maleimide reacts with cysteine residues to crop specific conjugates
Sulfo-SMCC is baptize soluble, so crosslinking can be done in physiologic solutions
High purity, apparent Sulfo-SMCC can be acclimated to actualize high-purity maleimide-activated derivatives
Cyclohexane arch confers added adherence to the maleimide accumulation authoritative Sulfo-SMCC the ideal crosslinking abettor for maleimide activation of proteins. Maleimide groups are abiding for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.
Applications for Sulfo-SMCC
Ideal crosslinker for agitator labeling of antibodies, both agitator action and antibiotic specificity can be preserved
Create specific bioconjugates via one- or two-step crosslinking reactions
Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens
Product Details:
Sulfo-SMCC is generally acclimated for the alertness of antibody-enzyme and hapten-carrier conjugates. In this blazon of conjugation, the NHS ester is reacted aboriginal with the antibody, balance crosslinking reagent removed and again the sulfhydryl-containing agitator atom is added. This two-step acknowledgment arrangement after-effects in accumulation of specific antibody-enzyme conjugates.
Sulfo-SMCC is the reagent of best for creating abiding maleimide-activated carrier proteins that will spontaneously acknowledge with sulfhydryls. In this application, the NHS ester of Sulfo-SMCC is reacted with lysine residues on the carrier protein, converting them to acknowledging maleimides. These almost abiding maleimide-activated intermediates may be lyophilized and stored for after alliance to a hapten.
Properties of Sulfo-SMCC
Molecular blueprint C16H17N2O9SNa
Molecular weight 436.37
Spacer arm breadth 8.3 Å (9 atom)
CAS Number 92921-24-9
Storage altitude -20°C, assure from moisture, use alone beginning solutions
Reactive groups: NHS ester, reacts with primary amines at pH 7.0-9.0
Maleimide, reacts with sulfhydryls at pH 6.5-7.5
Sulfo-SMCC Specifications. We accomplish Sulfo-SMCC to the accomplished blueprint to aftermath the a lot of specific bioconjugates and maleimide-activated proteins, ensure the candor of your abstracts and to accommodate you with the accomplished amount of crosslinking consistency. Each lot of Sulfo-SMCC is activated to accommodated the afterward minimum specifications.
Identity
IR browse shows alone peaks appropriate of the anatomy and anatomic groups of Sulfo-SMCC crosslinker
Purity ≥ 90% by quantitative NMR (the accomplished accepted for crosslinker purity)
Solubility ≥ 10 mg/ml in DI baptize
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