Lactobacillus rhamnosus Lysate Powder (Lactobacillus rhamnosus GG, LGG) are Lactobacillus , in the 1980s , pioneered by two scientists from the United States isolated healthy human intestine derived . In recent years , the bacteria become one of the most probiotics global concern . Large number of animal experiments and clinical trials show that , LGG can withstand animal digestive tract environment , and the ability of human and animal intestinal colonization , can modulate intestinal bacteria.
Lactic acid bacteria as probiotics have been widely applied to food and medicine , the physiological activity of lactic acid bacteria at home and abroad a large number of research reports. Recently, studies have reported that lactic acid bacteria with anti-aging , anti-oxidation and some other important physiological functions , is to develop an important resource for natural antioxidants . In this study, DPPH determination of four Lactobacillus rhamnosus B7, B8, B10, B44 antioxidant activity , analysis of cell-free extracts of each strain , whole-cell and cell lysate and other components of the DPPH scavenging and superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) activity , and enzyme inactivation strains before and after the DPPH scavenging components were compared .Then the test results , selected a relatively high antioxidant activity of Lactobacillus rhamnosus strain continued in vivo and in vitro antioxidant activity and research . According to alcoholism , the pro-oxidants and anti- oxidant balance destruction , leading to fat, protein and DNA oxidative damage and other biological macromolecules , thereby leading to cell damage in a mouse model theory to prove the design of Lactobacillus rhamnosus antioxidant activity .Analysis of mouse plasma alanine aminotransferase (ALT), lipopolysaccharide (LPS), superoxide dismutase (SOD), malondialdehyde (MDA) and tissue triglyceride (TG), free fatty acids and cholesterol levels to detect Lactobacillus rhamnosus strains of alcohol -induced oxidative stress protection. The use of ethanol and Lactobacillus rhamnosus B10 Caco-2 cells after treatment , the use of ethidium staining and dihydro- immunoblotting analysis of intracellular reactive oxygen species (ROS) and inducible nitric oxide synthase (NOS2) do trace detection analysis.
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