Wednesday, May 9, 2012

The characteristics of 9-Fluorenylmethyl carbazate


The adjustment of advancing a amoroso alternation sample as claimed in affirmation 1, wherein said labeled amoroso alternation has at atomic one hydrazide group-containing admixture called from those listed below, apprenticed to the abbreviation end thereof: 2-aminobenzhydrazide; 2-hydrazinobenzoic acid; benzylhydrazine; 5-Dimethylaminonaphthalene-1-sulfonyl hydrazine (Dansylhydrazine); 2-hydrazinopyridine; 9-fluorenylmethyl carbazate (Fmoc hydrazine); 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid, hydrazide; 2-(6,8-difluoro-7-hydroxy-4-methylcoumarin) acetohydrazide; 7-diethylaminocoumarin-3-carboxylic acid, hydrazide (DCCH); phenylhydrazine; 1-Naphthaleneacethydrazide; and phenylacetic hydrazide.
The adjustment of advancing a amoroso alternation sample as declared in any one of (1) to (12), wherein the labeled amoroso alternation has at atomic one hydrazide group-containing admixture called from those listed below, apprenticed to the abbreviation end thereof: [0035] 2-aminobenzhydrazide; 2-hydrazinobenzoic acid; benzylhydrazine; 5-Dimethylaminonaphthalene-1-sulfonyl hydrazine (Dansylhydrazine); 2-hydrazinopyridine; 9-fluorenylmethyl carbazate (Fmoc hydrazine); 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid, hydrazide; 2-(6,8-difluoro-7-hydroxy-4-methylcoumarin) acetohydrazide; 7-diethylaminocoumarin-3-carboxylic acid, hydrazide (DCCH); phenylhydrazine; 1-Naphthaleneacethydrazide; and phenylacetic hydrazide. 2-aminobenzhydrazide; 2-hydrazinobenzoic acid; benzylhydrazine; 5-dimethylaminonaphthalene-1-sulfonyl hydrazine (Dansylhydrazine); 2-hydrazinopyridine; 9-fluorenylmethyl carbazate (Fmoc hydrazine); 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid, hydrazide; 2-(6,8-difluoro-7-hydroxy-4-methylcoumarin) acetohydrazide; 7-diethylaminocoumarin-3-carboxylic acid, hydrazide(DCCH); phenylhydrazine; 1-Naphthaleneacethydrazide; and phenylacetic hydrazide.
A adjustment of advancing a amoroso alternation sample, for abbreviation unreacted labeling reagent in a sample band-aid absolute a labeled amoroso chain, includes (process 1) a action of bringing the sample band-aid absolute the labeled amoroso alternation into acquaintance with caked silica, so as to acquiesce the caked silica to adsorb a amoroso alternation component; (process 2) a action of abrasion the caked silica with a abrasion liquid; and (process 3) a action of bringing the caked silica into acquaintance with an eluant, so as to elute the adsorbed amoroso chain.
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