O’in 1 DNA Polymerase Premix

O’in 1 DNA Polymerase with Mg2+ is our high quality YEAtaq DNA Polymerase containing all the nucleotides and reagents necessary to perform a standard amplification reaction. It saves the time for preparing the master mix and reduces the risk of contamination from multiple pipetting steps.

Applications:Ready to use
Master mix
Suitable for economic screening

Components:
1× O’in 1 DNA Polymerase Premix10 mM KCl , 2 mM MgSO4•7H2O, 20 mM Tris-HCl (pH 8.8), 0.1% Triton X-100, 10 mM (NH4)2SO4 , 0.1 mg/ml BSA, 0.2 mM dNTP mix, 50U/ml YEAtaq DNA Polymerase, stabilizers.
2× O’in 1 DNA Polymerase PremixTwo folds of all the above reagents

Quality Controln:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.

About YEAtaq DNA Polymerase

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.

Applications:Screening
Primer extension
Amplification
Terminal dA tailing

Components:
YEAtaq DNA
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA
The reaction buffer is supplied as a 10× concentrate and should be diluted for use.

Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Quality Control:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.

Note:

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pre-stained Protein Markers

Pre-stained Protein Markers are suitable for monitoring protein separation during electrophoresis. It is a mixture of purified recombinant proteins covalently coupled to a blue colored chromophores and with the apparent molecular weight from 7 kDa to 240 kDa.

Features and Benefit:
Offers visualization of standard proteins without staining during electrophoresis or electrophoretic transfer onto membranes.
Ready-to-use, pre-diluted with a gel loading buffer for direct application onto polyacrylamide gels.

Recommendations for Loading:Apply 5 µl/well on mini gels and 10 ~ 15 µl/well on large gels.
For blots, apply 1 ~ 3 µl/well on mini gels and 2 ~ 6 µl/well on large gels..

Covalently coupled chromophores may affect the mobility of the standard proteins, and the apparent molecular weight may slightly shift. Pre-stained protein marker is good for approximate molecular weight determination. Each lot of pre-stained protein marker is calibrated against a precisely sized, unstained protein marker.

About YLOS Transformation Kit

YLOS Transformation Kit provides a simple and fast one-step/one-tube method to transform Yarrowia cells cultured in either solid agar or liquid broth. They were designed for various strains of Yarrowia lipolytica and vectors. Transformation efficiencies with Y. lipolytica will vary based on the yeast strain used, a linear or circular plasmid DNA, the efficiency of plasmid integration into the host chromosome, and the transformation procedure chosen. In general, the entire procedure may be completed in 60 min, and routinely provides a transformation efficiency of 103 ~ 105 transformants per mg of plasmid DNA.

Features and Benefits:
competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the oleaginous yeast
Simple (Mix → Heat shock → Plating)
Suitable for Yarrowia yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103 ~ 105 transformants /μg DNA

Buy YLOS Transformation Kit 

YLEX Expression Kit

YLEX Expression Kit based on INRA INAPG licensed patent provides an easy approach for cloning and expressing a gene of interest in the yeast, Yarrowia lipolytica . Using this kit, high level of heterologous protein may be expressed intracellularly or be secreted from the cell into medium by selecting the supplied expression vector pYLEX1 or pYLSC1.

Features and Benefits:

SafeIt was classified as GRAS (generally regarded as safe) by the US FDA (Food and Drug Administration)
Simple
A simple tool for expressing heterologous protein
Easy manipulation
Like E. coli and Saccharomyces cerevisiae
StableStrong stability in vectors and constructed plasmids
ReliableVectors integrated at the same site in genome
FlexibleBoth expression and secretion vector provided (Proteins may be expressed intracellularly or be secreted from the cell into medium)
High growth ability, High secretion capacity & High product yieldAdapted to high throughput screening and Industrial mass production of recombinant proteins
Less protein degradedNo extracellular protease synthesized by a special protease-deficient Yarrowia strain
Mass ProductionIndustrial mass production of recombinant proteins
Less hyperglycosylation
Able to perform post-translational processing of complex proteins, unlike Saccharomyces cerevisiae

SCOS Transformation Kit

SCOS Transformation Kit provides a simple and fast one-step / one-tube method for transforming the yeast, S. cerevisiae with a linear or circular plasmid DNA. The transformation efficiency varies based on the yeast strain used, the efficiency of plasmid incorporated into the host chromosome, the selection marker used and the transformation procedure. In general, the entire procedure can be completed in ONE hour, and routinely provides a transformation efficiency of 103~106 transformants / μg of plasmid DNA.

Features and benefits:
High throughput transformation
No competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the baker’s yeast
Simple (Mix → Heat shock → Plating)
Suitable for yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103~106 transformants /μg DNA

Buy SCOS Transformation Kit 

Deoxynucleotides

The enzyme DNA polymerase, which uses deoxynucleoside triphosphates as substrates, makes DNA. To ensure enough precursors for DNA synthesis, two reactions must occur. First, the 2′ position of the ribose ring of ribonucleotides must be reduced from a C‐OH to a C‐H before the nucleotides can be used for DNA synthesis. Secondly, the thymine ring must be made by addition of a methyl group to uridine.

The small protein thioredoxin supplies reducing equivalents to ribonucleotide reductase for the ribose ring reduction. Thioredoxin is itself reduced by another protein, thioredoxin reductase, a flavoprotein. Reduced glutathione can also carry reducing equivalents to ribonucleotide reductase. In both cases, the ultimate source of reducing equivalents is NADPH.

The regulation of ribonucleotide reductase is complex, with many feedback reactions used to keep the supplies of deoxynucleotides in balance. For example, dGTP and dTTP are feedback inhibitors of their own formation. Each is also an activator of the synthesis of the complementary nucleotide (dCDP or dADP), while dATP is an inhibitor of the reductions to make dADP, dCDP, dGDP, and dUDP. These control functions keep the supply of deoxynucleotides in balance, so that a roughly equivalent amount of each remains available for DNA synthesis.