RealStart DNA Polymerase Premix

RealStart DNA polymerase premix is a set of ultra-sensitive and convenient PCR product. It contains all the factors and loading dye* needed to perform PCR, including HotStart DNA polymerase、dNTPs、optimized buffers, etc. The only step it takes to perform PCR with RealStart DNA polymerase premix is to add the template and primers into the reaction mix. Since special HotStart DNA polymerase in the premix activates after heating, it reduces the risk of non-specific amplification when work with RealStart DNA polymerase premix at room temperature.

Features and Benefits:
Simple & Time-SavingAdd the template and primers into the RealStart DNA Polymerase
Less ContaminationReduce non-specific amplification caused by mispriming events that occur during setup and initial temperature increase.
High SensitivityThe sensitivity level is equivalent to that of real-time PCR
ConvenientAn excellent tool when w

orking with high quantities of samples
Sample SizeWork excellent for short DNA templates (size shorter than 600 bp)
Work well with yT&A Cloning Kit

Components:Hotstart Taq DNA polymerase
dNTPs mix (including dATP, dCTP, dGTP, dTTP)
7.5 mM MgCl2
Loading dye (including bromphenol blue)
One without loading dye. The other with loading dye.

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Quality Control:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.

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