YLEX Expression Kit based on INRA INAPG licensed patent provides an easy approach for cloning and expressing a gene of interest in the yeast, Yarrowia lipolytica . Using this kit, high level of heterologous protein may be expressed intracellularly or be secreted from the cell into medium by selecting the supplied expression vector pYLEX1 or pYLSC1.
Features and Benefits:
SafeIt was classified as GRAS (generally regarded as safe) by the US FDA (Food and Drug Administration)
Simple
A simple tool for expressing heterologous protein
Easy manipulation
Like E. coli and Saccharomyces cerevisiae
StableStrong stability in vectors and constructed plasmids
ReliableVectors integrated at the same site in genome
FlexibleBoth expression and secretion vector provided (Proteins may be expressed intracellularly or be secreted from the cell into medium)
High growth ability, High secretion capacity & High product yieldAdapted to high throughput screening and Industrial mass production of recombinant proteins
Less protein degradedNo extracellular protease synthesized by a special protease-deficient Yarrowia strain
Mass ProductionIndustrial mass production of recombinant proteins
Less hyperglycosylation
Able to perform post-translational processing of complex proteins, unlike Saccharomyces cerevisiae
Simple
A simple tool for expressing heterologous protein
Easy manipulation
Like E. coli and Saccharomyces cerevisiae
StableStrong stability in vectors and constructed plasmids
ReliableVectors integrated at the same site in genome
FlexibleBoth expression and secretion vector provided (Proteins may be expressed intracellularly or be secreted from the cell into medium)
High growth ability, High secretion capacity & High product yieldAdapted to high throughput screening and Industrial mass production of recombinant proteins
Less protein degradedNo extracellular protease synthesized by a special protease-deficient Yarrowia strain
Mass ProductionIndustrial mass production of recombinant proteins
Less hyperglycosylation
Able to perform post-translational processing of complex proteins, unlike Saccharomyces cerevisiae
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