YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.
Applications:Screening
Primer extension
Amplification
Terminal dA tailing
Primer extension
Amplification
Terminal dA tailing
Components:
YEAtaq DNA
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA
*The reaction buffer is supplied as a 10× concentrate and should be diluted for use.
YEAtaq DNA
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA
*The reaction buffer is supplied as a 10× concentrate and should be diluted for use.
Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.
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