The T&A cloning kit contains the T&A cloning vector and many
reagents needed for ligation. It is a convenient pack for cloning PCR
product generated using thermostable DNA polymerases, such as YEAtaq DNA
polymerase, which add a single terminal 3’-dA nucleotide overhang.
After ligation, the mixture can be used directly to transform into
competent cells (ECOS) or be purified first to achieve higher
transformation efficiency. The kit contains enough reagents for 20
reactions.
More T&A cloning kit .
No comments:
Post a Comment