YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.
Applications:Screening
Primer extension
Amplification
Terminal dA tailing
Primer extension
Amplification
Terminal dA tailing
Components:
YEAtaq DNA
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA
The reaction buffer is supplied as a 10× concentrate and should be diluted for use.
YEAtaq DNA
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA
The reaction buffer is supplied as a 10× concentrate and should be diluted for use.
Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.
Quality Control:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.
Note:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
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