JM109 is often used to select the recombinant DNA
using the activity of β-galactosidase (α -complementation) recovered
with lacZα peptide of the vector DNA and lacZΔM15 coded in F' episome in
this strain. This strain carries F' episome and it is useful for the
preparation of ssDNA as well as gene library construction and
subcloning.
This product, when used in combination with the rapid ligation
kit or T&A Cloning Kit becomes a powerful tool in cloning. The
ligation can be finished within 5 minutes then followed by 1-minute
transformation. The fast growth rate of JM109 allows colonies to show up
within 8 hours. Use this strain when you need to get the clone in
urgent.
ECOS 9-5 Competent Cells are chemically competent and have been
developed using a novel chemical preparation. ECOS technology makes the
cell highly efficient for DNA uptake.
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