GM2163 is an E. coliis both Dam– and Dcm–, so it is useful for
production of DNA to be cut with Dam or Dcm-sensitive restriction
enzymes. This strain is resistant to chloramphenicol. This strain is not
suitable for blue/white screening.
Genotype:
F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2
GM2163 Applications:
Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is resistant to chloramphenicol.
This strain is not suitable for blue/white screening.
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