Tuesday, November 5, 2013

ECOS 2163 Competent Cells Strain GM2163

GM2163 is an E. coliis both Dam– and Dcm–, so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is resistant to chloramphenicol. This strain is not suitable for blue/white screening.
Genotype:
F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2
GM2163 Applications:
Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is resistant to chloramphenicol.
This strain is not suitable for blue/white screening.

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