Tuesday, November 26, 2013

O’in 1 DNA Polymerase Premix

O’in 1 DNA Polymerase with Mg2+ is our high quality YEAtaq DNA Polymerase containing all the nucleotides and reagents necessary to perform a standard amplification reaction. It saves the time for preparing the master mix and reduces the risk of contamination from multiple pipetting steps.

Applications:Ready to use
Master mix
Suitable for economic screening
Components:
1× O’in 1 DNA Polymerase Premix
10 mM KCl , 2 mM MgSO4•7H2O, 20 mM Tris-HCl (pH 8.8), 0.1% Triton X-100, 10 mM (NH4)2SO4 , 0.1 mg/ml BSA, 0.2 mM dNTP mix, 50U/ml YEAtaq DNA Polymerase, stabilizers.
2× O’in 1 DNA Polymerase PremixTwo folds of all the above reagents
Quality Controln:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.

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