YLOS Transformation Kit provides a simple and fast one-step/one-tube method to transform Yarrowia cells cultured in either solid agar or liquid broth. They were designed for various strains of Yarrowia lipolytica and vectors. Transformation efficiencies with Y. lipolytica will vary based on the yeast strain used, a linear or circular plasmid DNA, the efficiency of plasmid integration into the host chromosome, and the transformation procedure chosen. In general, the entire procedure may be completed in 60 min, and routinely provides a transformation efficiency of 103 ~ 105 transformants per mg of plasmid DNA.
Features and Benefits:
competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the oleaginous yeast
Simple (Mix → Heat shock → Plating)
Suitable for Yarrowia yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103 ~ 105 transformants /μg DNA
competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the oleaginous yeast
Simple (Mix → Heat shock → Plating)
Suitable for Yarrowia yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103 ~ 105 transformants /μg DNA
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