HiFi DNA polymerase is ideal for economical amplification of DNA fragments, especially for high-fidelity PCR. High fidelity is achieved by an optimal blend of high performance YEAtaq DNA polymerase and a Pyrococcus proofreading (3'→5' exonuclease activity ) enzyme.
This formulation achieves greater yields with higher fidelity than standard DNA polymerases such as Taq. HiFi is a quality choice for sensitive applications.
We offer two types of HiFi DNA polymerase, one with dNTPs mix and the other without.
This formulation achieves greater yields with higher fidelity than standard DNA polymerases such as Taq. HiFi is a quality choice for sensitive applications.
We offer two types of HiFi DNA polymerase, one with dNTPs mix and the other without.
Applications:
Cloning
Blunt end amplification products
DNA expression
Mutation Analysis
Efficient & highly sensitive PCR use
Cloning
Blunt end amplification products
DNA expression
Mutation Analysis
Efficient & highly sensitive PCR use
Components:
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4 , 1 mg/ml BSA
*The reaction buffer is supplied as a 10× concentrate and should be diluted for use.
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4 , 1 mg/ml BSA
*The reaction buffer is supplied as a 10× concentrate and should be diluted for use.
Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.
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