About ECOS 21 Competent Cells Strain: BL-21 (DE3)

This strain provides high level of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. BL-21 is deficient in lon protease and the outer membrane protease, OmpT. The lack of these proteases reduces degradation of heterologous proteins expressed in the strain. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol” is recommended if high efficiency is desired.ECOS 21 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly .

About ECOSTM 10B Competent Cells Strain DH10B

 DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids. This strain is an MC1061 derivative, Streptomycin resistant. DH10B is suitable for the cloning of methylated cytosine or adenine containing DNA, for blue/white selection. While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence is published .

About Total RNA Extraction Kits a

The series of Total RNA Extraction Kits are specially designed for purification of total RNA from a variety of samples, such as bacteria, yeasts, plant tissues, animal tissues, cultured cells, fresh human whole blood, etc. The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase, and then RNA in chaotropic salt solutions binds to the glass fiber matrix in the columns. After washing off the contaminants, purified RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for plenty of applications.
Applications:
RT-PCR
cDNA synthesis
In vitro translation
Primer extension
Northern blotting
cDNA library construction
Quality Control:
The quality of Total RNA Extraction Kits is tested on a lot-to-lot basis.
Note:
For research use only. Not for use in diagnostic or therapeutic procedures.
RB Buffer contains chaotropic salt whichis a harmful and irritant agent. During operation, always wear a lab coat, disposable gloves, and protective goggles.
Use Conditions:
Sample Source: animal tissue, whole blood/buffy coat, cultured animal cells, gram+/– bacteria.
Sample Size: 30 mg for tissue, 107 animal cells, 300 μl of whole blood, 109 bacterial cells.

About PCR DNA Fragments Extraction Kits

PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments from agarose gel, PCR or other enzymatic reactions. The method uses a chaotropic salt, guanidine thiocyanante to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column or in each well of the plate. After washing off the contaminants, the purified DNA fragments are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation. Typical recoveries are 60 ~ 80% for gel extraction and 80 ~ 90% for PCR clean-up. The entire procedure can be completed in 20 minutes and the eluted DNA is ready to use in reaction digestion, ligation, PCR, and sequencing reaction.
Applications:
DNA sequencing
DNA library screening and analysis
 Restriction enzyme digestion
Ligation and transformation
Quality Control:
The quality of PCR DNA Fragments Extraction Kits is tested on a lot-to-lot basis.

About 96-Well SEQ Dye Clean Up Kit

96-Well SEQ Dye Clean Up Kit is designed for high-throughput purification of DNA sequencing reactions. Ultra filtration (UF) membrane is used to separate extremely small particles and dissolved molecules based on their size. Salts and unincorporated dye terminators are effectively removed from reaction mixtures without alcohol precipitation or gel filtration. The kit is compatible with ABI Prism BigDye, Beckman CEQ or Amersham DYEnamic ET Dye Terminator chemistries and can be used for manual filtration or with robotic handing systems. The entire procedure can be completed in 20 minutes.

About Viral Nucleic Acid Extraction Kits

Viral Nucleic Acid Extraction Kits are specially designed for purification of viral RNA or DNA from cell-free samples. With extraction method, DNA/RNA viruses are lysed quickly and efficiently by lysis buffer which is a highly concentrated solution of chaotropic salt. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the glass fiber matrix of the blood viral DNA/RNA binding column. Contaminations like salts, metabolites and soluble macromolecular cellular components are removed in washing steps. The nucleic acids can be eluted in low salt buffer or water and are ready-to-use in subsequent reactions.
The prepared nucleic acids are suitable for applications including real-time PCR/RT-PCR, automated fluorescent DNA sequencing, PCR, and many other enzymatic reactions. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assay. This protocol is recommended for parallel purification of viral RNA including HCV, HIV, and HTLV and viral DNA including HBV and CMV.
 More about Viral Nucleic Acid Extraction Kits

Plasmid Extraction Kits

Plasmid Extraction Kits are designed for rapid isolation of plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used to get clear cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the column. The contaminants are washed with ethanol wash buffer. In the final step, the purified plasmid DNA is eluted by low salt elution buffer or distilled water. The protocol does not require phenol extraction or alcohol precipitation. The entire procedure can be completed in 20 minutes and the purified plasmid DNA is ready for various applications.
Applications:
Plasmid DNA prearation
DNA sequencing
DNA library screening and analysis
Ligation and transformation
Transfection

Genomic DNA Extraction Kits

 Genomic DNA Extraction Kits provide a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from a wide range of samples such as plant tissue, animal tissue, whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes or cultured cells.
Applications:
PCR
Southern blotting
PADP/AFLP
Quality Control:
The quality of Genomic DNA Extraction Kits (Blood/Cultured Cell) is tested on a lot-to-lot basis.

G25 Oligo Clean Up Kit

The G25 Oligo Clean Up Kit consists of pre-packed Sephadax G25 pre-hydrated with double- distilled water. The columns are ideal for purification of synthesized oligonucleotides, end-labeled oligonucleotides, desalting and buffer exchange of PCR product. The G25 column can purify DNA fragments longer than 10 bases in length and low molecular weight material will be retained in the gel matrix of the column. The column is designed to purify DNA fragments > 10 bases only, therefore it is not recommended for PCR product primer removal.

About 96-Well PCR Clean Up Kit

96-Well PCR Clean Up Kit is designed for high-throughput purification of DNA fragments. Ultra filtration (UF) membrane is used to separate extremely small particles and dissolved molecules based on their size. Salts are effectively removed from reaction mixtures without the need for alcohol precipitation or gel filtration. The kits can be used for manual filtration or with robotic handling systems. The entire procedure can be completed in 20 minutes.

About YEA Protein Assay Kit

YEA Protein Assay Kit, based on the protein assay of Bradford, is a fast, simple and accurate procedure for detecting concentration of soluble protein. The principle of assay involves a differential color change of an acidic dye occurs at 595 nm when the dye is added to protein solution. The measurement could be achieved conveniently by using a regular spectrophotometer or microplate reader. When compared with a standard curve, it provides a relative measurement of protein concentration.
Features and Benefits:
Money saving
Protein Detection Reagent could be 1:4 diluted with dd-H2O.
Fast
Simply add Protein Detection Reagent and sample together.
Time saving
Mix and incubate at room temperature for 5 min.
The linear range of the assay for BSA is from 1 ~ 1000 g/ml.
Storage Conditions:
Store at room temperature (15 ~ 25°C)

About His-tag protein purification Mini Kit

His-tag protein purification Mini Kit offers a rapid and easy purification to purity 6X histidine-tagged proteins from bacteria. Pre-packed column with high quality resin and simple protocols speed up your work flow and make protein purification more reliable. The kits contain cell lysis and purification buffer (containing imidazloe) for 5 purifications. For unclarified and clarified cell lysates, the column also can provide suitable purifications by centrifugation or gravity flow. The design means that purification of His-tagged proteins is easier than ever before.
Feature and Benefits:
Histidine tagged protein purification
Protein binding capacity of column is 3mg~10mg
The column allows purification by centrifugation (for unclarified cell lysates) and gravity flow (for clarified cell lysates).
Contains cell lysis buffer and the capacity up to 100 ml bacterial culture per purification.

Features of Viral Nucleic Acid Extraction Kits

Viral Nucleic Acid Extraction Kits use of efficient, specific binding of nucleic acids and a unique centrifugal adsorption column buffer system, suitable from plasma, serum and other body fluids such as cell-free virus was isolated and purified samples of DNA or RNA, particularly in this kit to join the Carrier RNA, can trace from the system easily capture nucleic acid, with a convenient, high yield and good reproducibility.

Features:

• High purity: no presence of contaminants and inhibitors.
• Speed: within 30 minutes of DNA and RNA can be purified.
• Safety: No phenol / chloroform extraction, no ethidium bromide and cesium chloride, minimal exposure to hazardous substances.
• Scalable solution: dissolve the steps can be extended to fit the application.
• Versatility: the plasmid from 200ul, serum, urine, cell culture medium, supernatant, cell-free virus in body fluids extracted DNA.

The Introduction of YLOS Transformation Kit

The YLOS Transformation Kit allows an elementary and debauched one-step/one-tube know-how to metamorphose Yarrowia cellphones civilised in either self-coloured nutrient agar or limpid stock. They constituted configured as assorted distorts of Yarrowia lipolytica and transmitters. Translation efficiencies withe. lipolytica bequeath change supported the barm deform expended, a one-dimensional or annulated plasmid desoxyribonucleic acid, the efficiency from plasmid desegregation into the boniface chromosome, and the transmutation process picked out. Generally, the integral operation peradventure discharged inwards threescore Hokkianese, and habitually brings home the bacon a transmutation efficiency of 103 ~ one hundred five transformants per magnesium of plasmid desoxyribonucleic acid.

About ECOS 101 Competent Cells Strain DH5α

ECOS 101 Competent Cells Strain DH5α is the most popular strain worldwide. In addition to the application mentioned in the above chart, it is also useful for large plasmids and yeast two-hybrid system (up to 10~40 kb). Shuttle vector can be collected from total DNA extracted from Saccharomyces transformed strains.
  ECOS 101 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

ECOS 9-5 Competent Cells Strain JM109

JM109 is often used to select the recombinant DNA using the activity of β-galactosidase (α -complementation) recovered with lacZα peptide of the vector DNA and lacZΔM15 coded in F' episome in this strain. This strain carries F' episome and it is useful for the preparation of ssDNA as well as gene library construction and subcloning.
  This product, when used in combination with the rapid ligation kit  or T&A Cloning Kit  becomes a powerful tool in cloning. The ligation can be finished within 5 minutes then followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Use this strain when you need to get the clone in urgent.
  ECOS 9-5 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

The Introduction of Rapid Ligation Kit YEA T4

The Rapid Ligation Kit YEA T4 enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature(25°C).The Quick Ligation Kit is tested for transformation efficiency using the following protocol.LITMUS 28 vector is cut with either EcoR V (blunt) or Hind III (cohesive), treated with calf intestinal phosphatase and gel purified. Blunt inserts from a Hae III digest of φX174 DNA and cohesive inserts from a Hind III of digest of λ DNA are ligated into the respective vectors at a 3:1 insert:vector ratio using the Rapid Ligation Kit YEA T4 Protocol. Ligation products are transformed as described.

• T4 DNA Ligase Buffer should be thawed on the bench or in the palm of your hand, and not at 37°C (to prevent breakdown of ATP).
• Once thawed, T4 DNA Ligase Buffer should be placed on ice.
• BSA in the Ligase Buffer may precipitate when thawed. This will not affect ligation efficiency.
• Ligations can be performed in any of the four standa

The Introduction of T&A cloning vector

T & A cloning vector is very economical and convenient and fast cloning. Following ligation mixture can be directly used to transform HIT ™ or other competent cells or purified , in order to achieve higher transformation efficiency. Vector is a highly purified to reduce background clones.Vector is an efficient cloning of PCR products (TA Cloning) a special carrier. By these two vectors pUC18, pUC19 carrier converted in pUC18, pUC19 vector cloning site at the Xba I and Sal I recognition sites inserted between the EcoR V recognition sites , were digested with EcoR V reaction , and then on both sides of the 3 ‘end add “T” is made . Because most of the heat-resistant DNA polymerase for PCR reaction in the PCR products are 3 ‘end to add an “A” feature , so use these two products can greatly improve the connection of PCR products , cloning efficiency.
The clone intermediate vector, there is only a HindIII stand and does not have the BglII stand. After incorporation, will complement in insert T and a  cloning vector in the intermediate vector sequence, and causes these two new stands. This merit T& The intermediate vector makes the clone to be more economical and to be convenient.

About G50 Dye Terminator Removal Kit

The G50 Dye Terminator Removal Kit consists of pre-packed Sephadax G50 pre-hydrated with double-distilled water. The columns can be used for removing excess dye terminator, free nucleotides from sequencing and labeling reaction, desalting and for buffer exchange. The G50 column can purify DNA fragments longer than 20 bases in length with low molecular weight material retained in the gel matrix of the column. The column is designed to purify DNA fragments > 20 bases only, it is not suitable for PCR product primer removal.

About 96-Well SEQ Dye Clean Up Kit

96-Well SEQ Dye Clean Up Kit is designed for high-throughput purification of DNA sequencing reactions. Ultra filtration (UF) membrane is used to separate extremely small particles and dissolved molecules based on their size. Salts and unincorporated dye terminators are effectively removed from reaction mixtures without alcohol precipitation or gel filtration. The kit is compatible with ABI Prism BigDye, Beckman CEQ or Amersham DYEnamic ET Dye Terminator chemistries and can be used for manual filtration or with robotic handing systems. The entire procedure can be completed in 20 minutes.

About Pre-stained Protein Markers

Pre-stained Protein Markers are suitable for monitoring protein separation during electrophoresis. It is a mixture of purified recombinant proteins covalently coupled to a blue colored chromophores and with the apparent molecular weight from 7 kDa to 240 kDa.
Features and Benefit:
Offers visualization of standard proteins without staining during electrophoresis or electrophoretic transfer onto membranes.
Ready-to-use, pre-diluted with a gel loading buffer for direct application onto polyacrylamide gels.
Recommendations for Loading:
Apply 5 µl/well on mini gels and 10 ~ 15 µl/well on large gels.
For blots, apply 1 ~ 3 µl/well on mini gels and 2 ~ 6 µl/well on large gels.
More about Pre-stained Protein Markers .

About FaStain Protein Staining Solution

FaStain Protein Staining Solution provides a fast, convenient and sensitive method for staining proteins separated in both native and SDS-PAGE gels. The staining procedure is very straightforward — just simply incubates gels in the staining solution until protein bands developed. The procedure requires 15 minutes of washing and 15 minutes of staining. It takes only 30 minutes to detect as little as 5 ng of protein on a water-clear background.

About YLEX Expression Kit

YLEX Expression Kit based on INRA INAPG licensed patent provides an easy approach for cloning and expressing a gene of interest in the yeast, Yarrowia lipolytica . Using this kit, high level of heterologous protein may be expressed intracellularly or be secreted from the cell into medium by selecting the supplied expression vector pYLEX1 or pYLSC1.
YLEX Expression Kit was classified as GRAS (generally regarded as safe) by the US FDA (Food and Drug Administration).Adapted to high throughput screening and Industrial mass production of recombinant proteins

About SCOS Transformation Kit

 SCOS Transformation Kit provides a simple and fast one-step / one-tube method for transforming the yeast, S. cerevisiae with a linear or circular plasmid DNA. The transformation efficiency varies based on the yeast strain used, the efficiency of plasmid incorporated into the host chromosome, the selection marker used and the transformation procedure. In general, the entire procedure can be completed in ONE hour, and routinely provides a transformation efficiency of 103~106 transformants / μg of plasmid DNA.
 SCOS Transformation Kit's Features and benefits:
High throughput transformation
No competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the baker’s yeast
Simple (Mix → Heat shock → Plating)
Suitable for yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103~106 transformants /μg DNA

About Total RNA Extraction Kits

The series of Total RNA Extraction Kits are specially designed for purification of total RNA from a variety of samples, such as bacteria, yeasts, plant tissues, animal tissues, cultured cells, fresh human whole blood, etc. The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase, and then RNA in chaotropic salt solutions binds to the glass fiber matrix in the columns. After washing off the contaminants, purified RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for plenty of applications.
Applications:
RT-PCR
cDNA synthesis
In vitro translation
Primer extension
Northern blotting
cDNA library constructio

About YEA Protein Assay Kit

 YEA Protein Assay Kit, based on the protein assay of Bradford, is a fast, simple and accurate procedure for detecting concentration of soluble protein. The principle of assay involves a differential color change of an acidic dye occurs at 595 nm when the dye is added to protein solution. The measurement could be achieved conveniently by using a regular spectrophotometer or microplate reader. When compared with a standard curve, YEA Protein Assay Kit provides a relative measurement of protein concentration.

About YEAtaq DNA Polymerase

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.
YEAtaq DNA Polymerase's Applications:
Screening
Primer extension
Amplification
Terminal dA tailing

About Ling-Zhi Immunomodulatory Protein

 Ling-Zhi Immunomodulatory Protein is a novel oral immunomodulatory protein originally from Ganoderma tsugae. The efficacy of Ling-Zhi Immunomodulatory Protein has been verified by many lab tests. The oral administration of Ling-Zhi Immunomodulatory Protein could effectively improve immune function by promoting the immune cell proliferation, NK cell activity, phagocyte activity, cytokine secretion and antibody production. By enhancing the immune system, Ling-Zhi Immunomodulatory Protein can help human body to stay at the optimistic condition to fight diseases at all time.

About His-tag protein purification Mini Kit

His-tag protein purification Mini Kit offers a rapid and easy purification to purity 6X histidine-tagged proteins from bacteria. Pre-packed column with high quality resin and simple protocols speed up your work flow and make protein purification more reliable. The kits contain cell lysis and purification buffer (containing imidazloe) for 5 purifications. For unclarified and clarified cell lysates, the column also can provide suitable purifications by centrifugation or gravity flow. The design means that purification of His-tagged proteins is easier than ever before.
His-tag protein purification Mini Kit's Feature and Benefits:
Histidine tagged protein purification
Protein binding capacity of column is 3mg~10mg
The column allows purification by centrifugation (for unclarified cell lysates) and gravity flow (for clarified cell lysates).
Contains cell lysis buffer and the capacity up to 100 ml bacterial culture per purification.
Column can be regenerated by using stripping and charging buffer with kits

About Pre-stained Protein Markers

Pre-stained Protein Markers are suitable for monitoring protein separation during electrophoresis. Pre-stained Protein Markers  is a mixture of purified recombinant proteins covalently coupled to a blue colored chromophores and with the apparent molecular weight from 7 kDa to 240 kDa.
Features and Benefit:
Offers visualization of standard proteins without staining during electrophoresis or electrophoretic transfer onto membranes.
Ready-to-use, pre-diluted with a gel loading buffer for direct application onto polyacrylamide gels.

About Pre-stained Protein Markers

Pre-stained Protein Markers are suitable for monitoring protein separation during electrophoresis. It is a mixture of purified recombinant proteins covalently coupled to a blue colored chromophores and with the apparent molecular weight from 7 kDa to 240 kDa.
Features and Benefit:
Offers visualization of standard proteins without staining during electrophoresis or electrophoretic transfer onto membranes.
Ready-to-use, pre-diluted with a gel loading buffer for direct application onto polyacrylamide gels.

About YEA-His Spin Kit

YEA-His Spin Kit is specially designed to make protein purification as easy as DNA purification. Pre-packed columns with high quality resin and simple protocols speed up your work flow and make protein purifications more reliable. From single samples to high throughput protein purification, you can achieve faster time to results. The ready-to-use column immobilized metal ions affinity chromatography resin.
Histidine tagged protein purification has never been easier. The columns contain pre-packed Ni2+ Yealose resin which has a high specific binding affinity to 6× histidine residues. Simply bind, wash and elute high-purity protein with these ready-to-use columns. HIS Spin Kit gives exceptionally high-yield recovery.

The use of Total RNA Extraction Kits

The series of Total RNA Extraction Kits are specially designed for purification of total RNA from a variety of samples, such as bacteria, yeasts, plant tissues, animal tissues, cultured cells, fresh human whole blood, etc. The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase, and then RNA in chaotropic salt solutions binds to the glass fiber matrix in the columns. After washing off the contaminants, purified RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for plenty of applications.
Applications:
RT-PCR
cDNA synthesis
In vitro translation
Primer extension
Northern blotting
cDNA library construction

The use of YEAtaq DNA Polymerase

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.
Applications:
Screening
Primer extension
Amplification
Terminal dA tailing

Ling-Zhi Immunomodulatory Protein

A large amount of the novelimmunomodulatory protein Ling Zhi-8 (LZ-8) is synthesized in the mycelia of Ganoderma lucidum (Kino, K., Yamashita, A., Yamaoka, K., Watanabe, J., Tanaka, S., Ko, K., Shimizu, K., and Tsunoo, H. (1989) J. Biol. Chem. 264, 472-478). A cDNA and a gene for LZ-8 were isolated and characterized. The mixed oligonucleotide probes for LZ-8 cDNA were designed from the results of protein sequencing (Tanaka, S., Ko, K., Kino, K., Tsuchiya, K., Yamashita, A., Murasugi, A., Sakuma, S., and Tsunoo, H. (1989) J. Biol. Chem. 264, 16372-16377) and were used for screening the mycelial cDNA library. The nucleotide sequence of the cloned cDNA confirms the amino acid sequence of LZ-8 that was previously determined by protein sequencing. The clones containing the LZ-8 gene (lz-8) were obtained from the mycelial genomic DNA library using the cDNA probe. Two CCAAT-like sequences and one TATA box were found at the upstream region of the postulated transcription initiation site of lz-8. A small intron (61 nucleotides long) divided lz-8 into two exons at the 5'-untranslated region. The other characteristic sequences were also found around the postulated transcription initiation site and around the poly(A) additional site.

YLOS Transformation Kit

YLOS Transformation Kit provides a simple and fast one-step/one-tube method to transform Yarrowia cells cultured in either solid agar or liquid broth. They were designed for various strains of Yarrowia lipolytica and vectors. Transformation efficiencies with Y. lipolytica will vary based on the yeast strain used, a linear or circular plasmid DNA, the efficiency of plasmid integration into the host chromosome, and the transformation procedure chosen. In general, the entire procedure may be completed in 60 min, and routinely provides a transformation efficiency of 103 ~ 105 transformants per mg of plasmid DNA.

SCOS Transformation Kit

 SCOS Transformation Kit Saccharomyces cerevisiae (120 reactions) SCOS Transformation Buffer 12 ml Carrier DNA 0.6 ml DTT 0.2 YY014 plus SCOS Transformation Kit 1 YY014 and SCOS Transformation Kit Gene Cloning Products *SCOS Transformation Buffer, 12 ml *Carrier DNA, 0.6 ml *DTT Power, 0.2 g FYY101-120P or if you look for this products: Calcium Chloride E.coli Transformation Buffer, E.coli Transformation 5x250ml BC001 as new products in gentaur molecular products we have YLOS Transformation kit 1 YY025 about Rapid Transformation Kit 

SCOS Transformation Kit provides a simple and fast one-step / one-tube method for transforming the yeast, S. cerevisiae with a linear or circular plasmid DNA. The transformation efficiency varies based on the yeast strain used, the efficiency of plasmid incorporated into the host chromosome, the selection marker used and the transformation procedure. In general, the entire procedure can be completed in ONE hour, and routinely provides a transformation efficiency of 103~106 transformants / μg of plasmid DNA.

Features and benefits:
High throughput transformation
No competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the baker’s yeast
Simple (Mix → Heat shock → Plating)
Suitable for yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103~106 transformants /μg DNA