Plasmid Extraction Kits

Plasmid Extraction Kits are designed for rapid isolation of plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used to get clear cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the column. The contaminants are washed with ethanol wash buffer. In the final step, the purified plasmid DNA is eluted by low salt elution buffer or distilled water. The protocol does not require phenol extraction or alcohol precipitation. The entire procedure can be completed in 20 minutes and the purified plasmid DNA is ready for various applications.
Applications:
Plasmid DNA prearation
DNA sequencing
DNA library screening and analysis
Ligation and transformation
Transfection