The series of Total RNA Extraction Kits are specially designed for
purification of total RNA from a variety of samples, such as bacteria,
yeasts, plant tissues, animal tissues, cultured cells, fresh human whole
blood, etc. The method uses detergents and a chaotropic salt to lyse
cells and inactivate RNase, and then RNA in chaotropic salt solutions
binds to the glass fiber matrix in the columns. After washing off the
contaminants, purified RNA is eluted by RNase-free water. The entire
procedure can be completed within 1 hour and the purified RNA is ready
for plenty of applications.
Applications:
RT-PCR
cDNA synthesis
In vitro translation
Primer extension
Northern blotting
cDNA library constructio
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