The use of Total RNA Extraction Kits

The series of Total RNA Extraction Kits are specially designed for purification of total RNA from a variety of samples, such as bacteria, yeasts, plant tissues, animal tissues, cultured cells, fresh human whole blood, etc. The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase, and then RNA in chaotropic salt solutions binds to the glass fiber matrix in the columns. After washing off the contaminants, purified RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for plenty of applications.
Applications:
RT-PCR
cDNA synthesis
In vitro translation
Primer extension
Northern blotting
cDNA library construction