JM109 is often used to select the recombinant DNA using the activity of
β-galactosidase (α -complementation) recovered with lacZα peptide of the
vector DNA and lacZΔM15 coded in F' episome in this strain. This strain
carries F' episome and it is useful for the preparation of ssDNA as
well as gene library construction and subcloning.
This product, when used in combination with the rapid ligation
kit or T&A Cloning Kit becomes a powerful tool in cloning. The
ligation can be finished within 5 minutes then followed by 1-minute
transformation. The fast growth rate of JM109 allows colonies to show up
within 8 hours. Use this strain when you need to get the clone in
urgent.
ECOS 9-5 Competent Cells are chemically competent and have been
developed using a novel chemical preparation. ECOS technology makes the
cell highly efficient for DNA uptake.
No comments:
Post a Comment