The Introduction of Rapid Ligation Kit YEA T4

The Rapid Ligation Kit YEA T4 enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature(25°C).The Quick Ligation Kit is tested for transformation efficiency using the following protocol.LITMUS 28 vector is cut with either EcoR V (blunt) or Hind III (cohesive), treated with calf intestinal phosphatase and gel purified. Blunt inserts from a Hae III digest of φX174 DNA and cohesive inserts from a Hind III of digest of λ DNA are ligated into the respective vectors at a 3:1 insert:vector ratio using the Rapid Ligation Kit YEA T4 Protocol. Ligation products are transformed as described.

• T4 DNA Ligase Buffer should be thawed on the bench or in the palm of your hand, and not at 37°C (to prevent breakdown of ATP).
• Once thawed, T4 DNA Ligase Buffer should be placed on ice.
• BSA in the Ligase Buffer may precipitate when thawed. This will not affect ligation efficiency.
• Ligations can be performed in any of the four standa