The Introduction of T&A cloning vector

T & A cloning vector is very economical and convenient and fast cloning. Following ligation mixture can be directly used to transform HIT ™ or other competent cells or purified , in order to achieve higher transformation efficiency. Vector is a highly purified to reduce background clones.Vector is an efficient cloning of PCR products (TA Cloning) a special carrier. By these two vectors pUC18, pUC19 carrier converted in pUC18, pUC19 vector cloning site at the Xba I and Sal I recognition sites inserted between the EcoR V recognition sites , were digested with EcoR V reaction , and then on both sides of the 3 ‘end add “T” is made . Because most of the heat-resistant DNA polymerase for PCR reaction in the PCR products are 3 ‘end to add an “A” feature , so use these two products can greatly improve the connection of PCR products , cloning efficiency.
The clone intermediate vector, there is only a HindIII stand and does not have the BglII stand. After incorporation, will complement in insert T and a  cloning vector in the intermediate vector sequence, and causes these two new stands. This merit T& The intermediate vector makes the clone to be more economical and to be convenient.