About Viral Nucleic Acid Extraction Kits

Viral Nucleic Acid Extraction Kits are specially designed for purification of viral RNA or DNA from cell-free samples. With extraction method, DNA/RNA viruses are lysed quickly and efficiently by lysis buffer which is a highly concentrated solution of chaotropic salt. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the glass fiber matrix of the blood viral DNA/RNA binding column. Contaminations like salts, metabolites and soluble macromolecular cellular components are removed in washing steps. The nucleic acids can be eluted in low salt buffer or water and are ready-to-use in subsequent reactions.
The prepared nucleic acids are suitable for applications including real-time PCR/RT-PCR, automated fluorescent DNA sequencing, PCR, and many other enzymatic reactions. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assay. This protocol is recommended for parallel purification of viral RNA including HCV, HIV, and HTLV and viral DNA including HBV and CMV.
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