Thursday, October 31, 2013

About ECOSTM 10B Competent Cells Strain DH10B

 DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids. This strain is an MC1061 derivative, Streptomycin resistant. DH10B is suitable for the cloning of methylated cytosine or adenine containing DNA, for blue/white selection. While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence is published .

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