YLOS Transformation Kit provides a simple
and fast one-step/one-tube method to transform Yarrowia cells cultured in
either solid agar or liquid broth. They were designed for various strains of
Yarrowia lipolytica and vectors. Transformation efficiencies with Y. lipolytica
will vary based on the yeast strain used, a linear or circular plasmid DNA, the
efficiency of plasmid integration into the host chromosome, and the
transformation procedure chosen. In general, the entire procedure may be
completed in 60 min, and routinely provides a transformation efficiency of 103
~ 105 transformants per mg of plasmid DNA.
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