About PCR DNA Fragments Extraction Kits

PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments from agarose gel, PCR or other enzymatic reactions. The method uses a chaotropic salt, guanidine thiocyanante to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column or in each well of the plate. After washing off the contaminants, the purified DNA fragments are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation. Typical recoveries are 60 ~ 80% for gel extraction and 80 ~ 90% for PCR clean-up. The entire procedure can be completed in 20 minutes and the eluted DNA is ready to use in reaction digestion, ligation, PCR, and sequencing reaction.
Applications:
DNA sequencing
DNA library screening and analysis
 Restriction enzyme digestion
Ligation and transformation
Quality Control:
The quality of PCR DNA Fragments Extraction Kits is tested on a lot-to-lot basis.