O’in 1 DNA Polymerase Premix

O’in 1 DNA Polymerase with Mg2+ is our high quality YEAtaq DNA Polymerase containing all the nucleotides and reagents necessary to perform a standard amplification reaction. It saves the time for preparing the master mix and reduces the risk of contamination from multiple pipetting steps.

Applications:Ready to use
Master mix
Suitable for economic screening

Components:
1× O’in 1 DNA Polymerase Premix10 mM KCl , 2 mM MgSO4•7H2O, 20 mM Tris-HCl (pH 8.8), 0.1% Triton X-100, 10 mM (NH4)2SO4 , 0.1 mg/ml BSA, 0.2 mM dNTP mix, 50U/ml YEAtaq DNA Polymerase, stabilizers.
2× O’in 1 DNA Polymerase PremixTwo folds of all the above reagents

Quality Controln:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.

About YEAtaq DNA Polymerase

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.

Applications:Screening
Primer extension
Amplification
Terminal dA tailing

Components:
YEAtaq DNA
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA
The reaction buffer is supplied as a 10× concentrate and should be diluted for use.

Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Quality Control:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.

Note:

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pre-stained Protein Markers

Pre-stained Protein Markers are suitable for monitoring protein separation during electrophoresis. It is a mixture of purified recombinant proteins covalently coupled to a blue colored chromophores and with the apparent molecular weight from 7 kDa to 240 kDa.

Features and Benefit:
Offers visualization of standard proteins without staining during electrophoresis or electrophoretic transfer onto membranes.
Ready-to-use, pre-diluted with a gel loading buffer for direct application onto polyacrylamide gels.

Recommendations for Loading:Apply 5 µl/well on mini gels and 10 ~ 15 µl/well on large gels.
For blots, apply 1 ~ 3 µl/well on mini gels and 2 ~ 6 µl/well on large gels..

Covalently coupled chromophores may affect the mobility of the standard proteins, and the apparent molecular weight may slightly shift. Pre-stained protein marker is good for approximate molecular weight determination. Each lot of pre-stained protein marker is calibrated against a precisely sized, unstained protein marker.

About YLOS Transformation Kit

YLOS Transformation Kit provides a simple and fast one-step/one-tube method to transform Yarrowia cells cultured in either solid agar or liquid broth. They were designed for various strains of Yarrowia lipolytica and vectors. Transformation efficiencies with Y. lipolytica will vary based on the yeast strain used, a linear or circular plasmid DNA, the efficiency of plasmid integration into the host chromosome, and the transformation procedure chosen. In general, the entire procedure may be completed in 60 min, and routinely provides a transformation efficiency of 103 ~ 105 transformants per mg of plasmid DNA.

Features and Benefits:
competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the oleaginous yeast
Simple (Mix → Heat shock → Plating)
Suitable for Yarrowia yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103 ~ 105 transformants /μg DNA

Buy YLOS Transformation Kit 

YLEX Expression Kit

YLEX Expression Kit based on INRA INAPG licensed patent provides an easy approach for cloning and expressing a gene of interest in the yeast, Yarrowia lipolytica . Using this kit, high level of heterologous protein may be expressed intracellularly or be secreted from the cell into medium by selecting the supplied expression vector pYLEX1 or pYLSC1.

Features and Benefits:

SafeIt was classified as GRAS (generally regarded as safe) by the US FDA (Food and Drug Administration)
Simple
A simple tool for expressing heterologous protein
Easy manipulation
Like E. coli and Saccharomyces cerevisiae
StableStrong stability in vectors and constructed plasmids
ReliableVectors integrated at the same site in genome
FlexibleBoth expression and secretion vector provided (Proteins may be expressed intracellularly or be secreted from the cell into medium)
High growth ability, High secretion capacity & High product yieldAdapted to high throughput screening and Industrial mass production of recombinant proteins
Less protein degradedNo extracellular protease synthesized by a special protease-deficient Yarrowia strain
Mass ProductionIndustrial mass production of recombinant proteins
Less hyperglycosylation
Able to perform post-translational processing of complex proteins, unlike Saccharomyces cerevisiae

SCOS Transformation Kit

SCOS Transformation Kit provides a simple and fast one-step / one-tube method for transforming the yeast, S. cerevisiae with a linear or circular plasmid DNA. The transformation efficiency varies based on the yeast strain used, the efficiency of plasmid incorporated into the host chromosome, the selection marker used and the transformation procedure. In general, the entire procedure can be completed in ONE hour, and routinely provides a transformation efficiency of 103~106 transformants / μg of plasmid DNA.

Features and benefits:
High throughput transformation
No competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the baker’s yeast
Simple (Mix → Heat shock → Plating)
Suitable for yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103~106 transformants /μg DNA

Buy SCOS Transformation Kit 

Deoxynucleotides

The enzyme DNA polymerase, which uses deoxynucleoside triphosphates as substrates, makes DNA. To ensure enough precursors for DNA synthesis, two reactions must occur. First, the 2′ position of the ribose ring of ribonucleotides must be reduced from a C‐OH to a C‐H before the nucleotides can be used for DNA synthesis. Secondly, the thymine ring must be made by addition of a methyl group to uridine.

The small protein thioredoxin supplies reducing equivalents to ribonucleotide reductase for the ribose ring reduction. Thioredoxin is itself reduced by another protein, thioredoxin reductase, a flavoprotein. Reduced glutathione can also carry reducing equivalents to ribonucleotide reductase. In both cases, the ultimate source of reducing equivalents is NADPH.

The regulation of ribonucleotide reductase is complex, with many feedback reactions used to keep the supplies of deoxynucleotides in balance. For example, dGTP and dTTP are feedback inhibitors of their own formation. Each is also an activator of the synthesis of the complementary nucleotide (dCDP or dADP), while dATP is an inhibitor of the reductions to make dADP, dCDP, dGDP, and dUDP. These control functions keep the supply of deoxynucleotides in balance, so that a roughly equivalent amount of each remains available for DNA synthesis.

RealStart DNA Polymerase Premix

RealStart DNA polymerase premix is a set of ultra-sensitive and convenient PCR product. It contains all the factors and loading dye* needed to perform PCR, including HotStart DNA polymerase、dNTPs、optimized buffers, etc. The only step it takes to perform PCR with RealStart DNA polymerase premix is to add the template and primers into the reaction mix. Since special HotStart DNA polymerase in the premix activates after heating, it reduces the risk of non-specific amplification when work with RealStart DNA polymerase premix at room temperature.

Features and Benefits:
Simple & Time-SavingAdd the template and primers into the RealStart DNA Polymerase
Less ContaminationReduce non-specific amplification caused by mispriming events that occur during setup and initial temperature increase.
High SensitivityThe sensitivity level is equivalent to that of real-time PCR
ConvenientAn excellent tool when w

orking with high quantities of samples
Sample SizeWork excellent for short DNA templates (size shorter than 600 bp)
Work well with yT&A Cloning Kit

Components:Hotstart Taq DNA polymerase
dNTPs mix (including dATP, dCTP, dGTP, dTTP)
7.5 mM MgCl2
Loading dye (including bromphenol blue)
One without loading dye. The other with loading dye.

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Quality Control:Nuclease activity is not detected after incubation of 1 g /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 l reaction buffer for 18 hours at 37°C.

Buy RealStart DNA polymerase premix 

HiFi DNA polymerase

HiFi DNA polymerase is ideal for economical amplification of DNA fragments, especially for high-fidelity PCR. High fidelity is achieved by an optimal blend of high performance YEAtaq DNA polymerase and a Pyrococcus proofreading (3'→5' exonuclease activity ) enzyme.
This formulation achieves greater yields with higher fidelity than standard DNA polymerases such as Taq. HiFi is a quality choice for sensitive applications.
We offer two types of HiFi DNA polymerase, one with dNTPs mix and the other without.

Applications:
Cloning
Blunt end amplification products
DNA expression
Mutation Analysis
Efficient & highly sensitive PCR use

Components:
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4 , 1 mg/ml BSA
*The reaction buffer is supplied as a 10× concentrate and should be diluted for use.

Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Buy HiFi DNA polymerase 

About Accu DNA Polymerase

Accu DNA Polymerase is purified from an E. coli strain carrying a plasmid with a cloned gene encoding a Pyrococcus sp. DNA polymerase. The enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5’→3’ direction in the presence of Mg2+ at 70~80°C. Accu DNA Polymerase exhibits 3’→ 5’ exonuclease (proofreading) activity, but has no detectable 5’→ 3’ exonuclease activity.

Applications:

Cloning

Amplification
Mutation Analysis
Accurate polymerization

Components:
Storage Buffer
 50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4 , 1 mg/ml BSA.
 *The reaction buffer is supplied as a 10× concentrate and should be diluted for use.

Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C

Quality Control:Nuclease activity is not detected after incubation of g /Hind III DNA with 5 units of Accu DNA polymerase in 50 μl reaction buffer for 18 hours at 37°C.

Pre-stained Protein Markers

Pre-stained Protein Markers are suitable for monitoring protein separation during electrophoresis. It is a mixture of purified recombinant proteins covalently coupled to a blue colored chromophores and with the apparent molecular weight from 7 kDa to 240 kDa.

Features and Benefit:
Offers visualization of standard proteins without staining during electrophoresis or electrophoretic transfer onto membranes.
Ready-to-use, pre-diluted with a gel loading buffer for direct application onto polyacrylamide gels.

Recommendations for Loading:Apply 5 µl/well on mini gels and 10 ~ 15 µl/well on large gels.
For blots, apply 1 ~ 3 µl/well on mini gels and 2 ~ 6 µl/well on large gels..

Note:
Covalently coupled chromophores may affect the mobility of the standard proteins, and the apparent molecular weight may slightly shift. Pre-stained protein marker is good for approximate molecular weight determination. Each lot of pre-stained protein marker is calibrated against a precisely sized, unstained protein marker.

FaStain Protein Staining Solution

FaStain Protein Staining Solution provides a fast, convenient and sensitive method for staining proteins separated in both native and SDS-PAGE gels. The staining procedure is very straightforward — just simply incubates gels in the staining solution until protein bands developed. The procedure requires 15 minutes of washing and 15 minutes of staining. It takes only 30 minutes to detect as little as 5 ng of protein on a water-clear background.

Features and Benefits:
ConvenientA ready-to-use staining solution
SimpleOne-step, no destaining step required
Fast30 minutes for standard staining procedure (3 5-min washing* & 15-min staining)
SensitiveAs little as 5 ng of protein can be detected
Environmental & SafeNo menthol or acetic acid included
Coomassie Brilliant Blue dye based

Applications:For both native PAGE or SDS-PAGE gels
Excellent for densitometry
Compatible with mass spectrometry

Buy FaStain Protein Staining Solution 

YEAtaq DNA Polymerase

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.

Applications:Screening
Primer extension
Amplification
Terminal dA tailing

Components:
YEAtaq DNA
Storage Buffer50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers
10× Reaction Buffer*100 mM KCl, 20 mM MgSO4•7H2O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA
*The reaction buffer is supplied as a 10× concentrate and should be diluted for use.

Unit Definition:One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

Buy YEAtaq DNA Polymerase 

EZtime Real-Time PCR Premix

The EZtime Real-Time PCR Premix is a ready-to-use , 2X concentrated premix reagent  including Hotstart Taq、SYBR Green I、optimized reaction buffer and nucleotides for running quantitative real-time PCR , including qPCR and 2-step qRT-PCR , in the SYBR Green I detection format.

Applications:

Quantitative real-time PCR
Quantitative 2-step RT-PCR
uick and accurate detection and quantification of target gene through real- time PCR

Componets:Hotstart Taq DNA polymerase
SYBR Green real-time PCR Buffer
dNTP mix including dATP, dCTP, dGTP, dTTP
5mM MgCl

UniversAll Tissue PCR Kit

The UniversAllT Tissue PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from a wide range of biological samples and amplify targets of interest by PCR.
Simplesingle-step extraction of genomic DNA to PCR. The UniversAll Tissue PCR Kit offers a novel one-step UniversAll Extract buffer that eliminates the need for freezing of cells or tissues with liquid nitrogen, mechanical disruption, organic extraction, column DNA purification, or alcohol precipitation.
Fastcells or tissues to PCR in 10 minutes.
Convenient
This product includes our superior PCR enzymes and buffers for amplification directly from the extract for your convenience.
Procedure:
Genomic DNA is extracted from small amount of samples simply by incubation in UniversAllTM Extract buffer for 10 minutes at 95 °C. After a brief vortex, a portion of the DNA extract is then ready to be added directly to the supplied or your own optimized PCR mix.
Application:
Perfect for small or large scale PCR-based genotyping and quantitative PCR.

Rapid Ligation Kit YEA T4

 YEA T4 DNA ligase catalyzes the joining of two strands of DNA between the 5’–phosphate and the 3’–hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. Rapid ligation kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.

T&A Cloning Kit

The T&A cloning kit contains the T&A cloning vector and many reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3’-dA nucleotide overhang. After ligation, the mixture can be used directly to transform into competent cells (ECOS) or be purified first to achieve higher transformation efficiency. The kit contains enough reagents for 20 reactions.
More T&A cloning kit .

T&A cloning vector

   Refering to the picyure above,before the insert incorporate into the T&A cloning vector, there is only one HindIII site and no BglII site. After the incorporation, the T and A nucleotide on the insert will complement the sequence on the vector and generate these two new sites. This merit of T&A vector makes cloning more economic and convenient.

ECOS 9-5 Competent Cells Strain JM109

JM109 is often used to select the recombinant DNA using the activity of β-galactosidase (α -complementation) recovered with lacZα peptide of the vector DNA and lacZΔM15 coded in F' episome in this strain. This strain carries F' episome and it is useful for the preparation of ssDNA as well as gene library construction and subcloning.
   This product, when used in combination with the rapid ligation kit  or T&A Cloning Kit  becomes a powerful tool in cloning. The ligation can be finished within 5 minutes then followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Use this strain when you need to get the clone in urgent.
  ECOS 9-5 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

ECOS 101 Competent Cells Strain DH5α

ECOS 101 Competent Cells Strain DH5α is the most popular strain worldwide. In addition to the application mentioned in the above chart, it is also useful for large plasmids and yeast two-hybrid system (up to 10~40 kb). Shuttle vector can be collected from total DNA extracted from Saccharomyces transformed strains.
         ECOS 101 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

Glass Plating Beads

Sterilized glass plating beads (4 mm) provide an efficient and easy method to achieve optimal spreading on agar plates. The rolling action of the glass beads gently spread the E. coli solution (or any bacterial/fungal solution) to reach areas of the plate that are inaccessible to spread. Multiple plates containing beads and E. coli solution may be stacked and shaken simultaneously to increase spreading throughout and reduce cell stress. The beads may be recovered by washing with 70% ethanol, sterilized by autoclaving, followed by oven dried.
Method to Use: For regular plating of ECOS cells, add 5~10 beads immediately after pipetting 50~100 ml of transformed cells onto each plate. Move the plate back and forth to roll the beads on the surface and change the direction of shaking a few times to ensure even spreading. When the surface of the agar starts to dry on the plate (20~40 seconds), pour off the beads and the plates are ready for incubation.

ECOS 2163 Competent Cells Strain GM2163

GM2163 is an E. coliis both Dam– and Dcm–, so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is resistant to chloramphenicol. This strain is not suitable for blue/white screening.
Genotype:
F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2
GM2163 Applications:
Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is resistant to chloramphenicol.
This strain is not suitable for blue/white screening.

ECOS Blue Competent Cells Strain XL1 Blue

  This strain is the most popular for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are tetracycline resistant. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of miniprep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
   ECOS Blue Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

ECOS 21 Competent Cells

 This strain provides high level of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. BL-21 is deficient in lon protease and the outer membrane protease, OmpT. The lack of these proteases reduces degradation of heterologous proteins expressed in the strain. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol” is recommended if high efficiency is desired.
 ECOS 21 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly

ECOS 9-5 Competent Cells Strain JM109

JM109 is often used to select the recombinant DNA using the activity of β-galactosidase (α -complementation) recovered with lacZα peptide of the vector DNA and lacZΔM15 coded in F' episome in this strain. This strain carries F' episome and it is useful for the preparation of ssDNA as well as gene library construction and subcloning.
 This product, when used in combination with the rapid ligation kit  or T&A Cloning Kit  becomes a powerful tool in cloning. The ligation can be finished within 5 minutes then followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Use this strain when you need to get the clone in urgent.
ECOS 9-5 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

ECOS 2163 Competent Cells Strain GM2163

GM2163 is an E. coliis both Dam– and Dcm–, so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is resistant to chloramphenicol. This strain is not suitable for blue/white screening.
Genotype:
F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2
GM2163 Applications:
Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is resistant to chloramphenicol.
This strain is not suitable for blue/white screening.