ECOS 101 Competent Cells Strain DH5α

ECOS 101 Competent Cells Strain DH5α is the most popular strain worldwide. In addition to the application mentioned in the above chart, it is also useful for large plasmids and yeast two-hybrid system (up to 10~40 kb). Shuttle vector can be collected from total DNA extracted from Saccharomyces transformed strains.
         ECOS 101 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

Glass Plating Beads

Sterilized glass plating beads (4 mm) provide an efficient and easy method to achieve optimal spreading on agar plates. The rolling action of the glass beads gently spread the E. coli solution (or any bacterial/fungal solution) to reach areas of the plate that are inaccessible to spread. Multiple plates containing beads and E. coli solution may be stacked and shaken simultaneously to increase spreading throughout and reduce cell stress. The beads may be recovered by washing with 70% ethanol, sterilized by autoclaving, followed by oven dried.
Method to Use: For regular plating of ECOS cells, add 5~10 beads immediately after pipetting 50~100 ml of transformed cells onto each plate. Move the plate back and forth to roll the beads on the surface and change the direction of shaking a few times to ensure even spreading. When the surface of the agar starts to dry on the plate (20~40 seconds), pour off the beads and the plates are ready for incubation.

ECOS 2163 Competent Cells Strain GM2163

GM2163 is an E. coliis both Dam– and Dcm–, so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is resistant to chloramphenicol. This strain is not suitable for blue/white screening.
Genotype:
F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2
GM2163 Applications:
Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is resistant to chloramphenicol.
This strain is not suitable for blue/white screening.

ECOS Blue Competent Cells Strain XL1 Blue

  This strain is the most popular for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are tetracycline resistant. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of miniprep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
   ECOS Blue Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

ECOS 21 Competent Cells

 This strain provides high level of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. BL-21 is deficient in lon protease and the outer membrane protease, OmpT. The lack of these proteases reduces degradation of heterologous proteins expressed in the strain. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol” is recommended if high efficiency is desired.
 ECOS 21 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly

ECOS 9-5 Competent Cells Strain JM109

JM109 is often used to select the recombinant DNA using the activity of β-galactosidase (α -complementation) recovered with lacZα peptide of the vector DNA and lacZΔM15 coded in F' episome in this strain. This strain carries F' episome and it is useful for the preparation of ssDNA as well as gene library construction and subcloning.
 This product, when used in combination with the rapid ligation kit  or T&A Cloning Kit  becomes a powerful tool in cloning. The ligation can be finished within 5 minutes then followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Use this strain when you need to get the clone in urgent.
ECOS 9-5 Competent Cells are chemically competent and have been developed using a novel chemical preparation. ECOS technology makes the cell highly efficient for DNA uptake.

ECOS 2163 Competent Cells Strain GM2163

GM2163 is an E. coliis both Dam– and Dcm–, so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is resistant to chloramphenicol. This strain is not suitable for blue/white screening.
Genotype:
F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2
GM2163 Applications:
Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is resistant to chloramphenicol.
This strain is not suitable for blue/white screening.