Friday, October 11, 2013

About YEA-His Spin Kit

YEA-His Spin Kit is specially designed to make protein purification as easy as DNA purification. Pre-packed columns with high quality resin and simple protocols speed up your work flow and make protein purifications more reliable. From single samples to high throughput protein purification, you can achieve faster time to results. The ready-to-use column immobilized metal ions affinity chromatography resin.
Histidine tagged protein purification has never been easier. The columns contain pre-packed Ni2+ Yealose resin which has a high specific binding affinity to 6× histidine residues. Simply bind, wash and elute high-purity protein with these ready-to-use columns. HIS Spin Kit gives exceptionally high-yield recovery.

Thursday, October 10, 2013

The use of Total RNA Extraction Kits


The series of Total RNA Extraction Kits are specially designed for purification of total RNA from a variety of samples, such as bacteria, yeasts, plant tissues, animal tissues, cultured cells, fresh human whole blood, etc. The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase, and then RNA in chaotropic salt solutions binds to the glass fiber matrix in the columns. After washing off the contaminants, purified RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for plenty of applications.
Applications:
RT-PCR
cDNA synthesis
In vitro translation
Primer extension
Northern blotting
cDNA library construction

The use of YEAtaq DNA Polymerase

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium. The enzyme is in a recombinant form expressed in E. coli. It is able to withstand repeated heating to 95°C without significant loss of activity.
We offer two types of YEAtaq DNA polymerase– one with dNTPs mix and the other without.
Applications:
Screening
Primer extension
Amplification
Terminal dA tailing

Wednesday, October 9, 2013

Ling-Zhi Immunomodulatory Protein


A large amount of the novelimmunomodulatory protein Ling Zhi-8 (LZ-8) is synthesized in the mycelia of Ganoderma lucidum (Kino, K., Yamashita, A., Yamaoka, K., Watanabe, J., Tanaka, S., Ko, K., Shimizu, K., and Tsunoo, H. (1989) J. Biol. Chem. 264, 472-478). A cDNA and a gene for LZ-8 were isolated and characterized. The mixed oligonucleotide probes for LZ-8 cDNA were designed from the results of protein sequencing (Tanaka, S., Ko, K., Kino, K., Tsuchiya, K., Yamashita, A., Murasugi, A., Sakuma, S., and Tsunoo, H. (1989) J. Biol. Chem. 264, 16372-16377) and were used for screening the mycelial cDNA library. The nucleotide sequence of the cloned cDNA confirms the amino acid sequence of LZ-8 that was previously determined by protein sequencing. The clones containing the LZ-8 gene (lz-8) were obtained from the mycelial genomic DNA library using the cDNA probe. Two CCAAT-like sequences and one TATA box were found at the upstream region of the postulated transcription initiation site of lz-8. A small intron (61 nucleotides long) divided lz-8 into two exons at the 5'-untranslated region. The other characteristic sequences were also found around the postulated transcription initiation site and around the poly(A) additional site.

YLOS Transformation Kit


YLOS Transformation Kit provides a simple and fast one-step/one-tube method to transform Yarrowia cells cultured in either solid agar or liquid broth. They were designed for various strains of Yarrowia lipolytica and vectors. Transformation efficiencies with Y. lipolytica will vary based on the yeast strain used, a linear or circular plasmid DNA, the efficiency of plasmid integration into the host chromosome, and the transformation procedure chosen. In general, the entire procedure may be completed in 60 min, and routinely provides a transformation efficiency of 103 ~ 105 transformants per mg of plasmid DNA.

Tuesday, October 8, 2013

SCOS Transformation Kit


 SCOS Transformation Kit Saccharomyces cerevisiae (120 reactions) SCOS Transformation Buffer 12 ml Carrier DNA 0.6 ml DTT 0.2 YY014 plus SCOS Transformation Kit 1 YY014 and SCOS Transformation Kit Gene Cloning Products *SCOS Transformation Buffer, 12 ml *Carrier DNA, 0.6 ml *DTT Power, 0.2 g FYY101-120P or if you look for this products: Calcium Chloride E.coli Transformation Buffer, E.coli Transformation 5x250ml BC001 as new products in gentaur molecular products we have YLOS Transformation kit 1 YY025 about Rapid Transformation Kit 
SCOS Transformation Kit provides a simple and fast one-step / one-tube method for transforming the yeast, S. cerevisiae with a linear or circular plasmid DNA. The transformation efficiency varies based on the yeast strain used, the efficiency of plasmid incorporated into the host chromosome, the selection marker used and the transformation procedure. In general, the entire procedure can be completed in ONE hour, and routinely provides a transformation efficiency of 103~106 transformants / μg of plasmid DNA.
Features and benefits:
High throughput transformation
No competent cells needed
An one-step, one-tube and 10 ~ 60-minute protocol for transforming the baker’s yeast
Simple (Mix → Heat shock → Plating)
Suitable for yeast cells from colonies, broth or any growth phase
Repeatable efficiency, always reach efficiency of 103~106 transformants /μg DNA

Tuesday, August 6, 2013

What is Zinc Picolinate

Zinc is one of the trace minerals that your body requires to maintain optimal health. Even though zinc is an abundant mineral, you can suffer from a zinc deficiency. If you have a zinc deficiency, doctors will typically recommend zinc supplements that support absorption by your body. Zinc picolinate is a type of zinc supplement that supports absorption. However, you should consult a medical professional prior to taking any zinc picolinate supplement.
Zinc picolinate is an acid form of zinc that your human body can more easily absorb than other forms of zinc. Absorption of zinc in your body is a complex process that involves the zinc passing through your intestinal membranes, into your blood stream and into your individual cells. Your body also requires zinc as a primary component to support metabolism in your body.